Journal: Oncology Letters
Article Title: RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma
doi: 10.3892/ol.2018.9543
Figure Lengend Snippet: RBBP7 has no effect on tumor growth and survival processes in ESCC. (A) TE1 and Eca109 cells were transfected with lentiviruses to knock down RBBP7 expression. Western blot assays were performed to verify the stable cell lines. (B) KYSE150 and YSE30 cells were transfected with lentiviruses to upregulate RBBP7 expression. Western blot assays were performed to verify the stable cell lines. (C) A plate colony formation assay was used to detect the colony formation rate. (D) Flow cytometry analysis was used to detect the cell apoptosis rate. Unpaired t tests were used in (C) and (D); data are shown as the mean values ± standard deviation. Scale bar, 1 cm. RBBP7, retinoblastoma-binding protein 7; ESCC, esophageal squamous cell carcinoma; ns, not significant; PI, propidium iodide.
Article Snippet: Human ESCC cell lines TE1, KYSE30, and KYSE150 were bought from Creative Bioarray (New York, USA) and Eca109 was obtained from the Committee of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China).
Techniques: Transfection, Knockdown, Expressing, Western Blot, Stable Transfection, Colony Assay, Flow Cytometry, Standard Deviation, Binding Assay